Vitafoods Asia 2019



25-26 September 2019  




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Allysulfides, garlic extracts used in dietary supplements

The main organosulfur compounds in whole garlic are the S-(+)-alkyl-L-cysteine sulfoxides (alliin, methiin, isoalliin and cycloalliin) and the γ-Glutamyl-S-alkyl-L-cysteines. Upon disrupting the cells by crushing the bulb, the enzyme alliinase comes in contact with the amino acid alliin, causing its rapid transformation into allicin. Allicin potentially becomes the main precursor of various other transformation compounds found in Regulid, our dietary supplement, such as allyl sulfides and vinyldithiins, when macerated in vegetable oils.





Allyl sulfides molecules.

Lipolytic Activity of Allylsulfides

Chemically rather simple molecules such as diallyldisulfide (DADS), methylallyltrisulfide (MATS) and diallyltrisulfide (DATS) present in Regulid seem to be connected with a rather extensive and quite complicated network of different biochemical formation and transformation, signalling and control pathways.


Several studies have shown that these organo-sulphur compounds were linked to different biological activities, including prevention of cardiovascular disease and cancer, antibacterial, antithrombotic, hypotensive and hypolipidaemic properties. bioXtract uses allyl sulfides to stabilize weight after a diet and avoid yo-yo dieting but also to help preventing metabolic syndrome.


An in vitro study run on human adipocytes [1] has demonstrated the lypolitic activity of DADS, one of Regulid active principals. Mature adipocytes isolated from visceral human fat were exposed to different concentrations of DADS (5 ÁM and 10 ÁM). DADS induced lipolysis in adipocytes (+80%, +87% for 5 and 10 ÁM respectively, compared to control).


Lypolitic activity of allyl sulfides.


Other biological properties


Allylsufides act through mechanisms such as modulation of drug-metabolizing enzymes, inhibition of cell proliferation, inhibition of genotoxicity, immune system enhancement and antioxidant activities.


An in vitro study was run on hepatic cells (Hep-G2) treated with different concentrations of sulfides (DADS and DATS) and allymercaptan (metabolites of the sulfides in blood). These molecules significantly inhibited the cholesterol biosynthesis as well as its excretion into the medium [2].

These results were confirmed by Liu et al. on primary rat hepatocytes [3]. The rat hepatocytes were treated with [2-14C]acetate as substrate for cholesterol synthesis in the presence or absence of garlic active principles.




[1] bioXtract, unpublished data.

[2] Cho BHS, Xu S. Effects of allyl mercaptan and various allium-derived compounds on cholesterol synthesis and secretion in Hep-G2 cells. Comparative Biochemistry and Physiology Part C. 2000; 126: 195-201.

[3] Liu L, Yeh YY. Inhibition of cholesterol biosynthesis by organosulfur compounds derived from garlic. Lipids. 2000; 35: 197-203.